Plasmid
Part:BBa_K783063:Design
Designed by: Traci Haddock Group: iGEM12_BostonU (2012-10-02)
Level 1 MoClo Destination Vector with EF
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to confirm the lacZ orientation was 5'->3' after cloning.
Source
We amplified the alpha lacZ fragment from pUC19 and used the BioBrick pSB1C3 backbone for our vector backbone.